Formation of Itaconic Acid from the Krebs Cycle Tricarboxylic Acids by Extracts of Aspergillus terreus.

The mechanism of itaconic acid formation by Aspergillus terreus. 2. The effect of substrates and inhibitors.

Relationship between morphology and itaconic acid production by Aspergillus terreus.

The morphology of filamentous fungi closely correlates with the productivity in submerged culture. Using itaconic acid (IA) production by Aspergillus terreus as a research model, the quantitative relationship between the growth form of A. terreus and IA production was investigated. IA fermentation was scaled up from shake flasks to a 7 L stirred tank bioreactor based on the quantitative relatio...

Direct production of itaconic acid from liquefied corn starch by genetically engineered Aspergillus terreus

BACKGROUND Itaconic acid is on the DOE (Department of Energy) top 12 list of biotechnologically produced building block chemicals and is produced commercially by Aspergillus terreus. However, the production cost of itaconic acid is too high to be economically competitive with the petrochemical-based products. Itaconic acid is generally produced from raw corn starch, including three steps: enzym...

Malonate inhibition of oxidations in the Krebs tricarboxylic acid cycle.

The inhibition of succinate oxidation by malonate is a well known phenomenon. Since the oxidation of succinate to fumarate is an integral part of the Krebs cycle of oxidations, it has been generally assumed that the inhibitory effect of malonate upon the oxidation of any member of the cycle is the result of the inhibition of the succinate to fumarate step. However, the present paper provides ev...

Expression of the Aspergillus terreus itaconic acid biosynthesis cluster in Aspergillus niger

BACKGROUND Aspergillus terreus is a natural producer of itaconic acid and is currently used to produce itaconic acid on an industrial scale. The metabolic process for itaconic acid biosynthesis is very similar to the production of citric acid in Aspergillus niger. However, a key enzyme in A. niger, cis-aconitate decarboxylase, is missing. The introduction of the A. terreus cadA gene in A. niger...